Glp-1 Regulates Proliferation of Glp-1 – Secreting Cells through a Feedback Mechanism
نویسنده
چکیده
.................................................................................................................................... 3 List of abbreviations ................................................................................................................... 4 Introduction .............................................................................................................................. 5 Diabetes mellitus ........................................................................................................................ 5 Incretins ...................................................................................................................................... 5 GIP ............................................................................................................................................. 5 GLP-1 ......................................................................................................................................... 6 GLP-1 Receptor .......................................................................................................................... 7 Physiological action of GLP-1 ................................................................................................... 8 GLP-1 in diabetes treatment ...................................................................................................... 8 The incretin effect of GLP-1 in type 2 diabetes .......................................................................... 9 GLP-1 and its stable analogues ................................................................................................. 9 Aim of the study ........................................................................................................................ 10 Materials and methods ............................................................................................................ 11 The Cells ................................................................................................................................... 11 Experimental methods .............................................................................................................. 11 Anti-diabetic agents .................................................................................................................. 11 3H-thymidine incorporation ..................................................................................................... 11 Immunocytochemistry with Ki67 staining ................................................................................ 11 Immunolabeling ........................................................................................................................ 12 Protein measurement BCA-assay ............................................................................................. 12 Western blot .............................................................................................................................. 12 Cell lysate preparation ............................................................................................................. 12 SDS-PAGE and Western blotting ............................................................................................. 12 Statistic methods ....................................................................................................................... 12 Results and Discussion ............................................................................................................ 14 Conclusion and future work ................................................................................................... 18 Acknowledgement .................................................................................................................... 18 References ................................................................................................................................ 19
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